[Plsgs] ABE Seminar - Kyung-Jin Jang, Harvard University

[Georgina Lambert]

From: Ibarra, Daniela - (dcastro) [mailto:dcastro at email.arizona.edu] 
Sent: Thursday, February 23, 2012 4:47 PM
To: Sonnenberg, Kerrie M - (kerrie); georgina at ag.arizona.edu
Subject: Please send to listserv: ABE Seminar - Kyung-Jin Jang, Harvard
University

 



 

Department of Agricultural and Bio-systems Engineering 

Graduate Seminar-ABE 696a

 

" In vivo-like microenvironments for renal tubular cells and

guided neurite outgrowth "

 

Abstract:

 

In vivo, cells respond to surrounding mechanical/chemical stimulations and
complex three-dimensional anisotropic environments. To understand these
mechanisms, cells need to be maintained in an in vivo-like environment that
reproduces key structural, functional, and mechanical properties of the
target organ. In this talk, I will describe two biomimetic microsystem that
reconstitutes critical functional aspects of the renal and nervous system.
To emulate the kidney tubular environment, microfluidic-based techniques
were used to recapitulate the complexity of the renal microenvironment in
vitro, including fluid shear stress and transepithelial chemical gradient.
The device included the integration of a polydimethyl siloxane (PDMS)
microfluidic channel to generate fluid shear stress, a porous membrane, and
a PDMS reservoir.  Culture of renal tubule cells in the microfluidic device
under fluid shear stress resulted in enhanced cell polarization,
cytoskeletal reorganization, and cilia formation.  In addition, to study the
complex morphogenetic event of neurite outgrowth, we used UV-assisted
capillary force lithography with polyurethane acrylate to construct
different topographic patterns. These nanotopographical patterns can provide
an insight into how neuronal growth cones can sense geometric ECM cues,
which possibly leads to new applications in tissue regeneration after nerve
injury as well as the treatment of neuropathological conditions. We also
have on-going work on human kidney proximal tubule-on-a-chip for more
predictive in vitro human kidney models for investigating absorption,
distribution, metabolism, excretion, and toxicological properties of new
chemical entities during the drug development process.  This novel system
may also provide a useful and cost-effective tool for studying
biotransformation profiles, renal pharmacology, renal drug transport and
toxicity relevant to the human kidney, and hence help to facilitate the drug
development process with a more human-relevant model.

Kyung-Jin Jang, PhD

Wyss Institute for Biologically Inspired Engineering, Harvard University

 


Monday, February 27, 2012


12:00 P.M. - 1:00 P.M.

Shantz 440

 

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