[Plsgs] Reminder: Please Join Us-3/26- Seminar- Laura Krings- "Quantitative analysis of tailocin killing activity in Pseudomonas syringae"

[Shinagawa, Keenia Alejandra Ibarra - (kshinagawa)]

Dear all,

Correction: Seminar is from 4-5pm today.


Please join us and support our Plant Science PhD Student: Laura Krings in her seminar presentation:

“Quantitative analysis of tailocin killing activity in Pseudomonas syringae”



Tuesday, March 26th, 2024

4-5PM

Marley 230



Zoom:

https://arizona.zoom.us/j/83044711714

(password: spls23)


Abstract: Bacteriocins are a narrow-range antimicrobial with potential as an alternative to antibiotics. Bacteriocins can be divided into three types: S, F, and R. F and R types are derived from the tails of bacteriophage, hence termed tailocins. R-type tailocins, especially those of pseudomonads, have been heavily studied and found to bind to Lipopolysaccharides (LPS) on gram negative bacteria. The structure of LPS is believed to influence the bacteria’s susceptibility or resistance to certain tailocins, with mutations in LPS biosynthesis genes leading to these changes by altering the structure of LPS. Generally to test a tailocins killing activity on a bacterium, a soft agar overlay assay is done, in which top agar is seeded with the target strain and an aliquot of tailocin is spotted on top. If the tailocin kills, a clear zone of killing is observed. This assay however has limitations in that it is qualitative and does not take into account a range in killing activity observed by different tailocins. Here we developed a quantitative assay using luminescence to measure the density of bacteria that have been treated with tailocins. Compared to controls, cultures treated with tailocins showed reduced growth and matched qualitative assays as well. Thus this assay appears to be a better measurement of tailocin killing activity and has the potential for use in other applications such as blocking assays to measure binding of tailocins to their receptors.


[cid:de8f3c67-c9fd-4341-94f4-6529ecf9b7f6]

Best,
Keenia
________________________________
From: Shinagawa, Keenia Alejandra Ibarra - (kshinagawa) <kshinagawa at arizona.edu>
Sent: Monday, March 25, 2024 2:34 PM
To: plsgs at list.cals.arizona.edu <Plsgs at list.cals.arizona.edu>; Plsfaculty <plsfaculty-bounces at list.cals.arizona.edu>
Cc: Bogner, David V - (bognerdv) <bognerdv at arizona.edu>
Subject: Please Join Us-3/26- Student Seminar- Laura Krings- "Quantitative analysis of tailocin killing activity in Pseudomonas syringae"


Dear all,


Please join us and support our Plant Science PhD Student: Laura Krings in her seminar presentation:

“Quantitative analysis of tailocin killing activity in Pseudomonas syringae”



Tuesday, March 26th, 2024

4-5PM

Marley 230



Zoom:

https://arizona.zoom.us/j/83044711714

(password: spls23)


[A flyer with text and images  Description automatically generated]



Thank you and see you there!



Keenia Shinagawa, M. Ed. she/her

Student Support | Graduate Coordinator

THE UNIVERSITY OF ARIZONA

School of Plant Sciences (SPLS)
College of Ag., Life & Environ. Sci (CALES)
Office: Forbes, 317
PO Box 210036 | Tucson, AZ 85721

O: (520) 621-1336
kshinagawa at arizona.edu<mailto:kshinagawa at arizona.edu>
www.arizona.edu<https://www.arizona.edu/>
facebook<https://www.facebook.com/UASchoolofPlantSciences> | twitter<https://twitter.com/UAPlantSciences> | instagram<https://www.instagram.com/uarizona> | linkedin<https://www.linkedin.com/school/the-university-of-arizona>

Student Resources:

CALS Life Management Counselors<https://cals.arizona.edu/life-management-services> | CALS Learning Lab<https://cals.arizona.edu/learning-lab> | Grad Path User Guides<https://grad.arizona.edu/gsas/gradpath/gradpath-user-guides>


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