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<span style="background-color: rgb(255, 255, 255);">Dear all,</span></div>
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<span style="background-color: rgb(255, 255, 0);"><b>Correction: Seminar is from 4-5pm today.</b></span></div>
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<p class="elementToProof" style="margin-top: 0px; margin-bottom: 0px;"><span style="font-family: "Times New Roman", serif; font-size: 14pt; color: black;">Please join us and support our Plant Science PhD Student:
<b>Laura Krings </b>in her seminar presentation:</span></p>
<p align="center" style="direction: ltr; text-indent: 0px; margin: 0in;"><span style="font-family: "Times New Roman", serif; font-size: 14pt; color: black;"><i>“Quantitative analysis of tailocin killing activity in Pseudomonas syringae”</i></span></p>
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<p align="center" style="direction: ltr; text-indent: 0px; margin: 0in;"><span style="font-family: "Times New Roman", serif; font-size: 14pt; color: black;"><b>Tuesday, March 26<sup>th</sup>, 2024</b></span></p>
<p align="center" style="direction: ltr; text-indent: 0px; margin: 0in;"><span style="font-family: "Times New Roman", serif; font-size: 14pt; color: black;"><b>4-5PM</b></span></p>
<p align="center" style="direction: ltr; text-indent: 0px; margin: 0in;"><span style="font-family: "Times New Roman", serif; font-size: 14pt; color: black;"><b>Marley 230</b></span></p>
<p align="center" style="direction: ltr; text-indent: 0px; margin: 0in;"><span style="font-family: "Times New Roman", serif; font-size: 14pt; color: rgb(33, 33, 33);"> </span></p>
<p align="center" style="direction: ltr; text-indent: 0px; margin: 0in;"><span style="font-family: "Times New Roman", serif; font-size: 14pt; color: black;">Zoom:</span></p>
<p align="center" style="direction: ltr; text-indent: 0px; margin: 0in;"><span style="font-family: "Times New Roman", serif; font-size: 14pt; color: rgb(0, 120, 215);"><a href="https://arizona.zoom.us/j/83044711714" id="OWAd2662a21-a386-66af-926d-29f8e2ea01a6" class="x_OWAAutoLink" data-auth="NotApplicable" data-loopstyle="linkonly" style="color: rgb(0, 120, 215); margin: 0px;">https://arizona.zoom.us/j/83044711714</a></span></p>
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<span style="font-family: "Times New Roman", serif; font-size: 14pt; color: black;">(password: spls23)</span></p>
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<span style="font-family: "Times New Roman", serif; font-size: 12pt; color: black;"><b><i>Abstract:</i></b><i>
</i></span><span style="font-family: "Times New Roman", serif; font-size: 12pt; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);"><i>Bacteriocins are a narrow-range antimicrobial with potential as an alternative to antibiotics. Bacteriocins can be
divided into three types: S, F, and R. F and R types are derived from the tails of bacteriophage, hence termed tailocins. R-type tailocins, especially those of pseudomonads, have been heavily studied and found to bind to Lipopolysaccharides (LPS) on gram negative
bacteria. The structure of LPS is believed to influence the bacteria’s susceptibility or resistance to certain tailocins, with mutations in LPS biosynthesis genes leading to these changes by altering the structure of LPS. Generally to test a tailocins killing
activity on a bacterium, a soft agar overlay assay is done, in which top agar is seeded with the target strain and an aliquot of tailocin is spotted on top. If the tailocin kills, a clear zone of killing is observed. This assay however has limitations in that
it is qualitative and does not take into account a range in killing activity observed by different tailocins. Here we developed a quantitative assay using luminescence to measure the density of bacteria that have been treated with tailocins. Compared to controls,
cultures treated with tailocins showed reduced growth and matched qualitative assays as well. Thus this assay appears to be a better measurement of tailocin killing activity and has the potential for use in other applications such as blocking assays to measure
binding of tailocins to their receptors.</i></span></p>
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<span style="font-family: "Times New Roman", serif; font-size: 12pt; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);"><i><img id="image_0" width="713" height="535" size="572136" contenttype="image/jpeg" style="width: 713px; height: 535px; margin-top: 0px; margin-bottom: 0px;" data-outlook-trace="F:1|T:1" src="cid:de8f3c67-c9fd-4341-94f4-6529ecf9b7f6"></i></span></p>
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<span style="background-color: rgb(255, 255, 255);">Best,</span></div>
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<span style="background-color: rgb(255, 255, 255);">Keenia</span></div>
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<b>From:</b> Shinagawa, Keenia Alejandra Ibarra - (kshinagawa) <kshinagawa@arizona.edu><br>
<b>Sent:</b> Monday, March 25, 2024 2:34 PM<br>
<b>To:</b> plsgs@list.cals.arizona.edu <Plsgs@list.cals.arizona.edu>; Plsfaculty <plsfaculty-bounces@list.cals.arizona.edu><br>
<b>Cc:</b> Bogner, David V - (bognerdv) <bognerdv@arizona.edu><br>
<b>Subject:</b> Please Join Us-3/26- Student Seminar- Laura Krings- "Quantitative analysis of tailocin killing activity in Pseudomonas syringae"</div>
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<span style="font-family: "Times New Roman", serif; font-size: 14pt; color: black;">Dear all,</span></p>
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<p align="center" style="direction: ltr; margin: 0in; font-family: Aptos, sans-serif;">
<span style="font-family: "Times New Roman", serif; font-size: 14pt; color: black;">Please join us and support our Plant Science PhD Student:
<b>Laura Krings </b>in her seminar presentation:</span></p>
<p align="center" style="direction: ltr; margin: 0in; font-family: Aptos, sans-serif;">
<span style="font-family: "Times New Roman", serif; font-size: 14pt; color: black;"><i>“Quantitative analysis of tailocin killing activity in Pseudomonas syringae”</i></span></p>
<p align="center" style="direction: ltr; margin: 0in; font-family: Aptos, sans-serif;">
<span style="font-family: "Times New Roman", serif; font-size: 14pt; color: rgb(33, 33, 33);"> </span></p>
<p align="center" style="direction: ltr; margin: 0in; font-family: Aptos, sans-serif;">
<span style="font-family: "Times New Roman", serif; font-size: 14pt; color: black;"><b>Tuesday, March 26<sup>th</sup>, 2024</b></span></p>
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<span style="font-family: "Times New Roman", serif; font-size: 14pt; color: black;"><b>4-5PM</b></span></p>
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<span style="font-family: "Times New Roman", serif; font-size: 14pt; color: black;"><b>Marley 230</b></span></p>
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<span style="font-family: "Times New Roman", serif; font-size: 14pt; color: black;">Zoom:</span></p>
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<span style="font-family: "Times New Roman", serif; font-size: 14pt; color: rgb(0, 120, 215);"><a href="https://arizona.zoom.us/j/83044711714" id="OWAb8eb5ba6-302c-f60b-e973-4701eea39e1e" class="x_OWAAutoLink" data-auth="NotApplicable" data-loopstyle="linkonly" style="color: rgb(0, 120, 215); margin-top: 0px; margin-bottom: 0px;">https://arizona.zoom.us/j/83044711714</a></span></p>
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<span style="font-family: "Times New Roman", serif; font-size: 14pt; color: black;">(password: spls23)</span></p>
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<span style="color: black;"><img alt="A flyer with text and images
Description automatically generated" id="x_Picture_x0020_1" width="687" height="515" style="width: 687px; height: 515px; margin-top: 0px; margin-bottom: 0px;" data-outlook-trace="F:2|T:2" src="cid:image001.jpg@01DA7EC1.195A30E0"></span></p>
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<span style="font-family: "Times New Roman", serif; color: black;">Thank you and see you there!</span></p>
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<p style="margin-top: 0px; margin-bottom: 0px;"><span style="font-family: "Edwardian Script ITC"; font-size: 18pt; color: rgb(23, 78, 134);"><b>Keenia Shinagawa, M. Ed.
</b></span><span style="font-family: "Times New Roman", serif; font-size: 10pt; color: rgb(73, 80, 87);">she/her</span></p>
<p style="margin-top: 0px; margin-bottom: 0px;"><span style="font-family: "Times New Roman", serif; font-size: 10pt; color: rgb(118, 62, 155); background-color: rgb(255, 255, 255);"><b><i>Student Support | Graduate Coordinator</i></b></span></p>
<p style="margin-top: 0px; margin-bottom: 0px;"><span style="font-family: "Times New Roman", serif; font-size: 9pt; color: rgb(200, 38, 19); background-color: rgb(255, 255, 255);"><b>THE UNIVERSITY OF ARIZONA</b></span></p>
<p style="text-align: left; background-color: rgb(255, 255, 255); margin: 0in;"><span style="font-family: "Times New Roman", serif; font-size: 10pt; color: rgb(111, 192, 64);"><i>School of Plant Sciences (SPLS)</i></span><span style="font-family: "Times New Roman", serif; font-size: 11pt; color: rgb(13, 13, 13);"><br>
</span><span style="font-family: "Times New Roman", serif; font-size: 10pt; color: rgb(12, 136, 42);"><i>College of Ag., Life & Environ. Sci (CALES)</i></span><span style="font-family: "Times New Roman", serif; font-size: 11pt; color: rgb(13, 13, 13);"><br>
</span><span style="font-family: "Times New Roman", serif; font-size: 10pt; color: rgb(81, 167, 249);">Office:
<b>Forbes, 317</b><br>
PO Box 210036 | Tucson, AZ 85721</span></p>
<p style="text-align: left; background-color: rgb(255, 255, 255); margin: 0in;"><span style="font-family: "Times New Roman", serif; font-size: 10pt; color: rgb(81, 167, 249);">O: (520) 621-1336</span><span style="font-family: "Times New Roman", serif; font-size: 11pt; color: rgb(13, 13, 13);"><br>
<a href="mailto:kshinagawa@arizona.edu" id="OWAdb0831e4-001a-be9f-7fd6-3a31054f67d2" class="OWAAutoLink" data-loopstyle="linkonly" style="margin: 0px;">kshinagawa@arizona.edu</a><br>
</span><span style="font-family: "Times New Roman", serif; font-size: 11pt; color: rgb(139, 0, 21);"><b><a href="https://www.arizona.edu/" id="OWAaeaafb79-f39a-8c4e-bfef-c8c7ba15972d" class="OWAAutoLink" data-auth="NotApplicable" data-loopstyle="linkonly" style="margin: 0px;">www.arizona.edu</a></b></span><span style="font-family: "Times New Roman", serif; font-size: 11pt; color: rgb(73, 80, 87);"><br>
</span><span style="font-family: "Times New Roman", serif; font-size: 11pt; color: rgb(139, 0, 21);"><a href="https://www.facebook.com/UASchoolofPlantSciences" id="OWAdb9cf906-98af-465c-b57a-e158a8bb48aa" class="OWAAutoLink" data-auth="NotApplicable" data-loopstyle="linkonly" style="margin: 0px;">facebook</a></span><span style="font-family: "Times New Roman", serif; font-size: 11pt; color: rgb(118, 118, 118);"> |
</span><span style="font-family: "Times New Roman", serif; font-size: 11pt; color: rgb(139, 0, 21);"><a href="https://twitter.com/UAPlantSciences" id="OWA8eb5b5e0-bfb7-bcb1-ea10-d10baca3661d" class="OWAAutoLink" data-auth="NotApplicable" data-loopstyle="linkonly" style="margin: 0px;">twitter</a></span><span style="font-family: "Times New Roman", serif; font-size: 11pt; color: rgb(118, 118, 118);"> |
</span><span style="font-family: "Times New Roman", serif; font-size: 11pt; color: rgb(139, 0, 21);"><a href="https://www.instagram.com/uarizona" id="OWA2b236900-2b62-eb68-9c52-76d4c97e3457" class="OWAAutoLink" data-auth="NotApplicable" data-loopstyle="linkonly" style="margin: 0px;">instagram</a></span><span style="font-family: "Times New Roman", serif; font-size: 11pt; color: rgb(118, 118, 118);"> |
</span><span style="font-family: "Times New Roman", serif; font-size: 11pt; color: rgb(139, 0, 21);"><a href="https://www.linkedin.com/school/the-university-of-arizona" id="OWAc5ee79b9-e689-abbc-7aa3-92622a4d4077" class="OWAAutoLink" data-auth="NotApplicable" data-loopstyle="linkonly" style="margin: 0px;">linkedin</a></span></p>
<p style="text-align: left; background-color: rgb(255, 255, 255); margin: 0in;"><span style="font-family: "Times New Roman", serif; font-size: 11pt; color: rgb(190, 91, 23);">Student Resources:</span></p>
<p style="text-align: left; background-color: rgb(255, 255, 255); margin: 0in;"><span style="font-family: "Times New Roman", serif; font-size: 11pt; color: rgb(0, 0, 0);"><a href="https://cals.arizona.edu/life-management-services" id="OWA15c03e01-32a0-1066-60bf-1b977bdc7926" class="OWAAutoLink" data-auth="NotApplicable" data-loopstyle="linkonly" style="margin: 0px;">CALS
Life Management Counselors</a> </span><span style="font-family: "Times New Roman", serif; font-size: 11pt; color: rgb(73, 80, 87);">|
</span><span style="font-family: "Times New Roman", serif; font-size: 11pt; color: rgb(0, 0, 0);"><a href="https://cals.arizona.edu/learning-lab" id="OWAafd7ac36-734c-6212-333b-92cc783c7590" class="OWAAutoLink" data-auth="NotApplicable" data-loopstyle="linkonly" style="margin: 0px;">CALS
Learning Lab</a> | <a href="https://grad.arizona.edu/gsas/gradpath/gradpath-user-guides" id="OWA3b628dca-1185-fd27-52bf-df67cb51dc4a" class="OWAAutoLink" title="https://grad.arizona.edu/gsas/gradpath/gradpath-user-guides" data-auth="NotApplicable" data-loopstyle="linkonly" style="margin-top: 0px; margin-bottom: 0px;">
Grad Path User Guides</a></span></p>
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