[Plsfaculty] Demonstration of Pressure Cycling Technology for extracting DNA, RNA, protein extraction from Plant & Soil Samples

[Barry Pryor]

FYI to all labs interested,
Sorry for such a late notice, but tommorrow I will host a demonstration by Jose
Lanuza of Pressure Biosciences of the new technique of extracting biomolecules
from complex environmental samples using pressure cycling technology (see email
below and visit their site at http://www.pressurebiosciences.com/).  Jose will
arrive at UofA at 1:00, set up the equipment in my lab, and then at 2:00 will
give a brief 20 min presentation in Marley 341H about PCT.  After the
presentation, we will go up to my lab and he will process whatever samples we
want for 1 hour.  Each sample will take about 5-10 minutes, so we can process
perhaps 10 samples.  Once processed, you can take your samples back to your
labs, run them on a gel to see if you like the results, and then discuss with
Jose the next day when he returns for some follow up discussions. What you will
need to do in advance of the demo is have your sample in your standard
extraction buffer.  The PCT equipment can handle a variety of types of samples
so whichever buffer works best for you, that's what you should use.  Give me a
call if you have questions about sample prep as I will be discussing with Jose
a little more today.  And let me know if someone in your lab will be attending
the demo so I can inform Jose of the expected number.  Thanks.

Barry



----- Forwarded message from bmpryor at u.arizona.edu -----
>
> Dear Dr. Pryor,
>

> Thank you for giving me the time to speak with you this afternoon about
> sample extraction in your laboratory.
>
> I am writing to update you on our revolutionary technology that utilizes
> Pressure Cycling Technology (PCT) for the extraction of biomolecules
> from complex matrices such as soil and plant material.
>
> Below are a series of links representing a few of the agriculture
> related applications that may be of interest.
> 1. Pressure Cycling Technology Sample Preparation System (PCT SPS)
> Improves Quantification of Pathogen DNA in Plants and Soil
> http://www.pressurebiosciences.com/downloads/posters/2007/Okub_PAG07_011
> 007_
> nl_rs.pdf
>
> 2. Improved extraction of Rhizoctonia and Pythium DNA from wheat roots
> and soil samples using pressure cycling technology
> http://www.pressurebiosciences.com/downloads/3rdparty-2007-12/PCT_CJPP-2
> 007.
> pdf
>
> 3. Extraction of DNA from Plant Tissue Using Pressure Cycling Technology
> (PCT)
> http://www.pressurebiosciences.com/downloads/app_notes/AN-10006.v1.pdf
>
>
> 4. Processing Bacillus Vegetative Cells and Spores Using Pressure
> Cycling Technology (PCT)
> http://www.pressurebiosciences.com/downloads/app_notes/AN-10007.v1.pdf
>
> 5. Extraction of RNA from Plant Tissue Using Pressure Cycling Technology
> (PCT)
> http://www.pressurebiosciences.com/downloads/app_notes/AN-10009.v1.pdf
>
>
> PCT effectively releases biomolecules using alternating cycles of high
> and low pressures. Sample is placed in specially designed, single-use
> processing containers (PULSE Tubes) and are then subjected to
> alternating cycles of high (up to 35,000 PSI) and ambient pressures in a
> pressure-generating instrument (Barocycler). Maximum and minimum
> pressures, the time at each pressure level, and the number of cycles is
> defined using a programmable controller. The reaction chamber of the
> Barocycler instrument is temperature controlled using a peripheral
> circulating water bath; therefore proteins and other biomolecules are
> not subject to denaturation. Safety features in the PCT technology were
> designed to significantly reduce the risk of exposure of pathogens to
> the researcher.
> There are a number of important differences between PCT and other
> currently used extraction methods such as:
>
> . Sonication
> . Bead Beating
> . Mortar & Pestle
> . French Press
> . Homogenization
>
> PCT offers many advantages including safety, reproducibility, speed,
> ease-of-use, and convenience. In addition, since PCT uses a unique
> extraction mechanism, it may be possible to reveal proteins, as well as
> other biomolecules, that are not readily released by conventional
> extraction methods. As a PCT user, you have the flexibility of using
> your own buffers or those we provide via our kits. If your downstream
> analysis requires detergent free conditions as is the case with Mass
> Spectrometry, we provide this capability via our platform.
>
> For more information on these and other application areas please visit
> our website, www.pressurebiosciences.com.
>
> If you are interested in discussing PCT or a demosntration, please let
> me know and I will follow-up with a phone call. I look forward to
> hearing back from you.
>
>
>
> By the way, I tried to get in touch wiht Purchasing but was unable to
> speak with Mr. Ted Masser, Assistant Director who handles vendor show at
> AU but I will try to call him again tomorrow
>
> Best regards,
>
> Jose' Q. Lanuza
> Director of Sales, Southwest Region
> Pressure BioSciences, Inc.
> (760) 402-6981
>
>
>
>
> <http://na3.salesforce.com/servlet/servlet.ImageServer?oid=00D500000007r
> bm&e
> sid=01850000009xQfS>
>
> ----- End forwarded message -----

----- End forwarded message -----