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<p class="MsoNormal"><b><span style="font-size:16.0pt">TODAY! <o:p></o:p></span></b></p>
<p class="MsoNormal"><b><span style="font-size:16.0pt">February 8, 4:00 PM<o:p></o:p></span></b></p>
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<p class="MsoNormal" style="margin-bottom:8.0pt;line-height:105%"><span style="font-size:14.0pt;line-height:105%">Join us T0day for the School of Plant Sciences Seminar. This event is only offered virtually, via Zoom.
<o:p></o:p></span></p>
<p class="MsoNormal" style="margin-bottom:8.0pt;line-height:105%"><span style="font-size:14.0pt;line-height:105%">Title: A Forward Genetic Screen to Identify Genes Involved in 1O2-induced Cell Death and Selective Chloroplast Degradation in the Arabidopsis Thaliana
Plastid Ferrochelatase Two Mutant<br>
Speaker: Matt Lemke, MCB PhD Student<br>
Day/Time: Tuesday, February 8, 4:00 pm<br>
Zoom Link: <a href="https://arizona.zoom.us/j/86531632716">https://arizona.zoom.us/j/86531632716</a>
<br>
Password: spls2022<br>
Host: Jesse Woodson<o:p></o:p></span></p>
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<b><span style="color:#333333;background:white">Abstract:</span></b><span style="color:#333333;background:white"><br>
Plants have evolved complex signaling mechanisms to sense and respond to reactive oxygen species (ROS) generated during abiotic stresses. In particular, chloroplasts naturally generate large amounts of ROS during photosynthesis, including singlet oxygen (<sup>1</sup>O<sub>2</sub>).
This localized <sup>1</sup>O<sub>2</sub> can damage the chloroplast and trigger quality control systems that lead to selective chloroplast degradation. This raises an intriguing question; How do plants sense chloroplast dysfunction and selectively degrade
damaged chloroplasts to sustain healthy chloroplast populations? <o:p></o:p></span></p>
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<span style="color:#333333">Forward genetic screens are a valuable tool to shed light on such processes. Activation-<span style="background:white">tagging is a method that involves transforming plants with a T-DNA that carries enhancer sequences that can lead
to the overexpression of nearby genes. </span></span><span style="color:black">Using this method to create secondary mutations in the
<i>fc2</i> mutant, an <i>Arabidopsis</i> mutant that expresses conditional enhancements of chloroplast degradation and cell death, we have identified eight dominant
<i>fc2</i> activation tagged suppressor (<i>fas</i>) mutants, all of which suppress conditional cell death in the adult stage. Preliminary characterization of these mutants has highlighted diverse processes that are affected and may be involved in
</span><sup><span style="color:#333333;background:white">1</span></sup><span style="color:#333333;background:white">O<sub>2</sub>-induced cell death and chloroplast degradation or confer general stress tolerance to overcome excess
<sup>1</sup>O<sub>2</sub> accumulation. Further c</span><span style="color:black">haracterization of these mutants will help define the intricate mechanisms involved in
<sup>1</sup>O<sub>2</sub>-mediated chloroplast quality control (CQC) or highlight indirect systems that allow plants to tolerate abiotic stresses.<o:p></o:p></span></p>
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<b><span style="color:black">Bio:<o:p></o:p></span></b></p>
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<span style="color:black">Matt is from Colorado and completed his undergraduate degree in Molecular and Cellular Biology at the University of Arizona. His work with the Woodson lab involves the use of a forward genetic approach, activation tagging, in a suppressor
screen to investigate the molecular mechanisms involved in <sup>1</sup>O<sub>2</sub>-induced chloroplast quality control. He is also exploring a potential role for microautophagy as a mechanism in the vacuolar transport of damaged chloroplasts in CQC.<o:p></o:p></span></p>
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<span style="color:black">In his free time, Matt enjoys hiking, cinema, photography, and traveling as much as possible. <o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><b><span style="font-size:12.0pt;color:black">Jennifer Garcia, </span></b><b><span style="font-size:10.0pt;color:black;border:none windowtext 1.0pt;padding:0in">CGMP, DES</span></b><span style="font-size:12.0pt;color:black"><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:9.0pt;color:black;border:none windowtext 1.0pt;padding:0in">She/her/hers<br>
School of Plant Sciences<br>
1140 E. South Campus Dr., Forbes 303<o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:9.0pt;color:black">Office: 520-621-9728<o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:9.0pt;color:black;border:none windowtext 1.0pt;padding:0in">Cell: 520-444-8153 (text preferred)</span><span style="font-size:12.0pt;color:black"><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:9.0pt;color:black;border:none windowtext 1.0pt;padding:0in"><a href="mailto:jennyj@arizona.edu">jennyj@arizona.edu</a></span><span style="font-size:12.0pt;color:black"><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white;vertical-align:baseline"><span style="font-size:12.0pt;color:black"><a href="https://cals.arizona.edu/spls/home"><span style="font-size:10.0pt;color:blue;border:none windowtext 1.0pt;padding:0in">https://cals.arizona.edu/spls/home</span></a><o:p></o:p></span></p>
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<p class="MsoNormal"><i><span style="font-size:9.0pt;color:#C00000">Working on campus in Forbes 303 – Tuesdays, Wednesdays (morning), & Thursdays
<o:p></o:p></span></i></p>
<p class="MsoNormal"><i><span style="font-size:9.0pt;color:#C00000">Working remote Mondays, Wednesdays (afternoons) & Fridays
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