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<div style="direction: ltr;">Hi all,</div>
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<div style="direction: ltr;">It works well when it works:) The NEB site (and available software tools like Geneious) have good modules to help design the overhangs.
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<div style="direction: ltr;">Need a blunt cutting site on plasmid is all, and about 50bp overhangs on insert.<span id="ms-outlook-ios-cursor"></span></div>
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<div style="direction: ltr;">Key for the process is that you’re going to want to spend money for (or create) really really good competent cells bc it’s a low frequency thing.
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<div style="direction: ltr;">Dave</div>
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<div class="ms-outlook-ios-signature">Get <a href="https://aka.ms/o0ukef">Outlook for iOS</a></div>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> Plsfaculty <plsfaculty-bounces@list.cals.arizona.edu> on behalf of Galbraith, David W - (galbrait) <galbrait@arizona.edu><br>
<b>Sent:</b> Thursday, September 24, 2020 9:36:38 AM<br>
<b>To:</b> Plsfaculty@list.cals.arizona.edu <Plsfaculty@list.cals.arizona.edu><br>
<b>Cc:</b> David Galbraith <galbraith@arizona.edu><br>
<b>Subject:</b> [Plsfaculty] Something completely different</font>
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<p class="x_MsoNormal">Hi all: </p>
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<p class="x_MsoNormal">Does anyone have practical experience with Gibson Assembly? Thanks for any information!</p>
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<p class="x_MsoNormal">David </p>
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