[Plantsci] SPLS Tuesday Seminar –February 11 - Md Nafis Ul Alam, School of Plant Sciences, University of Arizona

[Xiong, Zhongguo - (zxiong)]

Dear all,

Please join us in Marley 230<https://map.arizona.edu/107> on Tuesday, February 11, at 4:00 PM for a seminar presented by Md Nafis Ul Alam, School of Plant Sciences, University of Arizona.

Zoom link: https://arizona.zoom.us/j/84253432688  (password: SPLS2025).
Refreshments will be provided in the Marley Lobby at 3:30 PM.

Title: Universal orthologs infer deep phylogenies and improve genome quality assessments

Abstract: Universal single-copy orthologs are the most stable components of genomes. Although they are routinely used for studying evolutionary histories and assessing new assemblies, current methods do not incorporate information from available genomic data. Here, we first determine the influence of evolutionary history on universal gene content in plants, fungi and animals. We find that across 11,098 genomes comprising 2,606 taxonomic groups, 215 groups significantly vary from their respective lineages in terms of their BUSCO (Benchmarking Universal Single Copy Orthologs) completeness. Additionally, 169 groups display an elevated complement of duplicated orthologs likely as an artifact of whole genome duplication events. Secondly, we investigate the extent of taxonomic congruence in BUSCO whole-genome phylogenies. For 275 suitable families out of 543 tested, sites evolving at higher rates produce up to 23.84% more taxonomically concordant, and up to 32.25% less terminally variable phylogenies compared to lower-rate sites. We find topological differences between BUSCO concatenated and coalescent trees to be marginal and conclude that higher rate sites from concatenated alignments produce the most congruent and least variable phylogenies. Finally, we show that BUSCO misannotations can lead to misrepresentations of assembly quality. To overcome this issue, we filter a Curated set of BUSCOs (CUSCOs) that provide up to 6.99% fewer false positives compared to the standard BUSCO search and introduce novel methods for comparing assemblies using BUSCO synteny. Overall, we highlight the importance of considering evolutionary histories during assembly evaluations and release the UniPhy software toolkit that reconstructs consistent phylogenies and reports phylogenetically informed assembly assessments.


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Zhongguo Xiong
School of Plant Sciences       BIO5 Institute
BIO5                           Email: zxiong at arizona.edu<mailto:zxiong at arizona.edu>
                               Phone: (520)-621-9869
Forbes 303, P.O. box 210036    Fax: (520)-621-7186
University of Arizona
Tucson, AZ 85721-0036          http://ag.arizona.edu/~zxiong
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